Mouse Assay:Article Title: An intercellular signaling pathway in the mouse retina connects Kv2.1, GLT-1, and nitric oxide synthase 1 to optic nerve regeneration
Article Snippet: .. Mice were euthanized at 14 days after NC and perfused transcardially with 0.9% saline and 4% paraformaldehyde (PFA; Sigma #441244) dissolved in 1X phosphate buffered saline (PBS) prepared from 10X PBS, (Bio-Rad cat# 1610780). .. Mice were euthanized at 14 days after NC and perfused transcardially with 0.9% saline and 4% paraformaldehyde (PFA; Sigma #441244) dissolved in 1X phosphate buffered saline (PBS) prepared from 10X PBS, (Bio-Rad cat# 1610780).
Saline:Article Title: An intercellular signaling pathway in the mouse retina connects Kv2.1, GLT-1, and nitric oxide synthase 1 to optic nerve regeneration
Article Snippet: .. Mice were euthanized at 14 days after NC and perfused transcardially with 0.9% saline and 4% paraformaldehyde (PFA; Sigma #441244) dissolved in 1X phosphate buffered saline (PBS) prepared from 10X PBS, (Bio-Rad cat# 1610780). .. Mice were euthanized at 14 days after NC and perfused transcardially with 0.9% saline and 4% paraformaldehyde (PFA; Sigma #441244) dissolved in 1X phosphate buffered saline (PBS) prepared from 10X PBS, (Bio-Rad cat# 1610780).
Article Title: miR-31-mediated local translation at the mitotic spindle is important for early development.
Article Snippet: .. Three 15-min Phosphate 822 Buffered Saline 0.1%Triton (PBST) (10X PBS; Bio-Rad, Hercules, CA) washes were 823 performed. .. 720 Injection solutions contained 20% sterile glycerol, 2 mg/mL 10,000 MW FITC 721 lysine charged dextran (ThermoFisher Scientific, Waltham, MA), and miR-31 LNA 722 power inhibitor, miR-124 LNA power inhibitor, control MASO, or Fascin translational-723 blocking MASO.
cDNA Synthesis:Article Title: Stable, neuron-specific gene expression in the mouse brain
Article Snippet: .. 10X PBS (1610780EDU, BioRad) 10X TBS (1706435EDU, BioRad) Tween-20 10% Solution (1610781EDU, BioRad) Ketamine (VINB-KETO-70121, Covetrus) Xylazine (AH0006P, Rampun) Heparin (H3393-100KU, Sigma) Syringe (701RN, Hamilton) Needles (Customized part #7803–07, Hamilton, 30G/15 mm/point 4/20° angle) VANNAS spring scissors (S11002-08, RWD) Small spatula 4% paraformaldehyde (J19943.K2, Life Technology Corporation) Trizol reagent (15,596,018, Life Technology Corporation) Ethanol 200 Proof (C933N40, Thomas Scientific) Isopropanol (BP26181, Thermofisher Scientific) First-strand cDNA synthesis master mix 4X (MB6008, ScienCell) Primers (IDT, see Table ) Powerup SYBR Green Master Mix (A25778, Applied Biosystems) PCR plates reader (Thermofisher Scientific) Pipettes and tips Guanidine-HCL (G4505, Sigma) Urea (U0631, Sigma) SDS (151–21-3, Bioworld) PBS-T (0.1%Tween-20 in PBS) 1% Casein in PBS or 5% non-fat milk in PBS (M-0842, Labscientific) O.C.T. compound (4583, Sakura) DAPI-prolonged diamond in mounting medium (P36962, Invitrogen) 4X Laemmli sample buffer (1,610,747, BioRad) Halt protease and phosphatase inhibitor cocktails (PI78446, Thermofisher scientific) Primary and secondary antibodies (see Table ) PM2500 protein marker (PM25002112201-1, ExcelBand) MES-SDS running buffer (NP0002, Invitrogen) HyPure Molecular Biology Grade Water (SH30538.03, VWR) Chloroform (LC130402, LabChem) MicroAmp Optical adhesive film (4,311,971, Applied Biosystems) Superfrost Plus Microscope Slides (22–037-246, Fisherbrand) NuPAGE 4–12% Bis–Tris Gel (NP0335B0X, Invitrogen) iBlot 2 NC Mini Stacks (IB23002, Invitrogen) Triton X100 (97,063–864, VWR) 2-Mercaptoethanol (M3148, Sigma) 15 ml and 50 ml falcon tubes 1.5 ml eppendorf tubes Surgical fine forceps (RWD Life science) Surgical scissors (RWD Life science) ..
SYBR Green Assay:Article Title: Stable, neuron-specific gene expression in the mouse brain
Article Snippet: .. 10X PBS (1610780EDU, BioRad) 10X TBS (1706435EDU, BioRad) Tween-20 10% Solution (1610781EDU, BioRad) Ketamine (VINB-KETO-70121, Covetrus) Xylazine (AH0006P, Rampun) Heparin (H3393-100KU, Sigma) Syringe (701RN, Hamilton) Needles (Customized part #7803–07, Hamilton, 30G/15 mm/point 4/20° angle) VANNAS spring scissors (S11002-08, RWD) Small spatula 4% paraformaldehyde (J19943.K2, Life Technology Corporation) Trizol reagent (15,596,018, Life Technology Corporation) Ethanol 200 Proof (C933N40, Thomas Scientific) Isopropanol (BP26181, Thermofisher Scientific) First-strand cDNA synthesis master mix 4X (MB6008, ScienCell) Primers (IDT, see Table ) Powerup SYBR Green Master Mix (A25778, Applied Biosystems) PCR plates reader (Thermofisher Scientific) Pipettes and tips Guanidine-HCL (G4505, Sigma) Urea (U0631, Sigma) SDS (151–21-3, Bioworld) PBS-T (0.1%Tween-20 in PBS) 1% Casein in PBS or 5% non-fat milk in PBS (M-0842, Labscientific) O.C.T. compound (4583, Sakura) DAPI-prolonged diamond in mounting medium (P36962, Invitrogen) 4X Laemmli sample buffer (1,610,747, BioRad) Halt protease and phosphatase inhibitor cocktails (PI78446, Thermofisher scientific) Primary and secondary antibodies (see Table ) PM2500 protein marker (PM25002112201-1, ExcelBand) MES-SDS running buffer (NP0002, Invitrogen) HyPure Molecular Biology Grade Water (SH30538.03, VWR) Chloroform (LC130402, LabChem) MicroAmp Optical adhesive film (4,311,971, Applied Biosystems) Superfrost Plus Microscope Slides (22–037-246, Fisherbrand) NuPAGE 4–12% Bis–Tris Gel (NP0335B0X, Invitrogen) iBlot 2 NC Mini Stacks (IB23002, Invitrogen) Triton X100 (97,063–864, VWR) 2-Mercaptoethanol (M3148, Sigma) 15 ml and 50 ml falcon tubes 1.5 ml eppendorf tubes Surgical fine forceps (RWD Life science) Surgical scissors (RWD Life science) ..
Polymerase Chain Reaction:Article Title: Stable, neuron-specific gene expression in the mouse brain
Article Snippet: .. 10X PBS (1610780EDU, BioRad) 10X TBS (1706435EDU, BioRad) Tween-20 10% Solution (1610781EDU, BioRad) Ketamine (VINB-KETO-70121, Covetrus) Xylazine (AH0006P, Rampun) Heparin (H3393-100KU, Sigma) Syringe (701RN, Hamilton) Needles (Customized part #7803–07, Hamilton, 30G/15 mm/point 4/20° angle) VANNAS spring scissors (S11002-08, RWD) Small spatula 4% paraformaldehyde (J19943.K2, Life Technology Corporation) Trizol reagent (15,596,018, Life Technology Corporation) Ethanol 200 Proof (C933N40, Thomas Scientific) Isopropanol (BP26181, Thermofisher Scientific) First-strand cDNA synthesis master mix 4X (MB6008, ScienCell) Primers (IDT, see Table ) Powerup SYBR Green Master Mix (A25778, Applied Biosystems) PCR plates reader (Thermofisher Scientific) Pipettes and tips Guanidine-HCL (G4505, Sigma) Urea (U0631, Sigma) SDS (151–21-3, Bioworld) PBS-T (0.1%Tween-20 in PBS) 1% Casein in PBS or 5% non-fat milk in PBS (M-0842, Labscientific) O.C.T. compound (4583, Sakura) DAPI-prolonged diamond in mounting medium (P36962, Invitrogen) 4X Laemmli sample buffer (1,610,747, BioRad) Halt protease and phosphatase inhibitor cocktails (PI78446, Thermofisher scientific) Primary and secondary antibodies (see Table ) PM2500 protein marker (PM25002112201-1, ExcelBand) MES-SDS running buffer (NP0002, Invitrogen) HyPure Molecular Biology Grade Water (SH30538.03, VWR) Chloroform (LC130402, LabChem) MicroAmp Optical adhesive film (4,311,971, Applied Biosystems) Superfrost Plus Microscope Slides (22–037-246, Fisherbrand) NuPAGE 4–12% Bis–Tris Gel (NP0335B0X, Invitrogen) iBlot 2 NC Mini Stacks (IB23002, Invitrogen) Triton X100 (97,063–864, VWR) 2-Mercaptoethanol (M3148, Sigma) 15 ml and 50 ml falcon tubes 1.5 ml eppendorf tubes Surgical fine forceps (RWD Life science) Surgical scissors (RWD Life science) ..
Marker:Article Title: Stable, neuron-specific gene expression in the mouse brain
Article Snippet: .. 10X PBS (1610780EDU, BioRad) 10X TBS (1706435EDU, BioRad) Tween-20 10% Solution (1610781EDU, BioRad) Ketamine (VINB-KETO-70121, Covetrus) Xylazine (AH0006P, Rampun) Heparin (H3393-100KU, Sigma) Syringe (701RN, Hamilton) Needles (Customized part #7803–07, Hamilton, 30G/15 mm/point 4/20° angle) VANNAS spring scissors (S11002-08, RWD) Small spatula 4% paraformaldehyde (J19943.K2, Life Technology Corporation) Trizol reagent (15,596,018, Life Technology Corporation) Ethanol 200 Proof (C933N40, Thomas Scientific) Isopropanol (BP26181, Thermofisher Scientific) First-strand cDNA synthesis master mix 4X (MB6008, ScienCell) Primers (IDT, see Table ) Powerup SYBR Green Master Mix (A25778, Applied Biosystems) PCR plates reader (Thermofisher Scientific) Pipettes and tips Guanidine-HCL (G4505, Sigma) Urea (U0631, Sigma) SDS (151–21-3, Bioworld) PBS-T (0.1%Tween-20 in PBS) 1% Casein in PBS or 5% non-fat milk in PBS (M-0842, Labscientific) O.C.T. compound (4583, Sakura) DAPI-prolonged diamond in mounting medium (P36962, Invitrogen) 4X Laemmli sample buffer (1,610,747, BioRad) Halt protease and phosphatase inhibitor cocktails (PI78446, Thermofisher scientific) Primary and secondary antibodies (see Table ) PM2500 protein marker (PM25002112201-1, ExcelBand) MES-SDS running buffer (NP0002, Invitrogen) HyPure Molecular Biology Grade Water (SH30538.03, VWR) Chloroform (LC130402, LabChem) MicroAmp Optical adhesive film (4,311,971, Applied Biosystems) Superfrost Plus Microscope Slides (22–037-246, Fisherbrand) NuPAGE 4–12% Bis–Tris Gel (NP0335B0X, Invitrogen) iBlot 2 NC Mini Stacks (IB23002, Invitrogen) Triton X100 (97,063–864, VWR) 2-Mercaptoethanol (M3148, Sigma) 15 ml and 50 ml falcon tubes 1.5 ml eppendorf tubes Surgical fine forceps (RWD Life science) Surgical scissors (RWD Life science) ..
Adhesive:Article Title: Stable, neuron-specific gene expression in the mouse brain
Article Snippet: .. 10X PBS (1610780EDU, BioRad) 10X TBS (1706435EDU, BioRad) Tween-20 10% Solution (1610781EDU, BioRad) Ketamine (VINB-KETO-70121, Covetrus) Xylazine (AH0006P, Rampun) Heparin (H3393-100KU, Sigma) Syringe (701RN, Hamilton) Needles (Customized part #7803–07, Hamilton, 30G/15 mm/point 4/20° angle) VANNAS spring scissors (S11002-08, RWD) Small spatula 4% paraformaldehyde (J19943.K2, Life Technology Corporation) Trizol reagent (15,596,018, Life Technology Corporation) Ethanol 200 Proof (C933N40, Thomas Scientific) Isopropanol (BP26181, Thermofisher Scientific) First-strand cDNA synthesis master mix 4X (MB6008, ScienCell) Primers (IDT, see Table ) Powerup SYBR Green Master Mix (A25778, Applied Biosystems) PCR plates reader (Thermofisher Scientific) Pipettes and tips Guanidine-HCL (G4505, Sigma) Urea (U0631, Sigma) SDS (151–21-3, Bioworld) PBS-T (0.1%Tween-20 in PBS) 1% Casein in PBS or 5% non-fat milk in PBS (M-0842, Labscientific) O.C.T. compound (4583, Sakura) DAPI-prolonged diamond in mounting medium (P36962, Invitrogen) 4X Laemmli sample buffer (1,610,747, BioRad) Halt protease and phosphatase inhibitor cocktails (PI78446, Thermofisher scientific) Primary and secondary antibodies (see Table ) PM2500 protein marker (PM25002112201-1, ExcelBand) MES-SDS running buffer (NP0002, Invitrogen) HyPure Molecular Biology Grade Water (SH30538.03, VWR) Chloroform (LC130402, LabChem) MicroAmp Optical adhesive film (4,311,971, Applied Biosystems) Superfrost Plus Microscope Slides (22–037-246, Fisherbrand) NuPAGE 4–12% Bis–Tris Gel (NP0335B0X, Invitrogen) iBlot 2 NC Mini Stacks (IB23002, Invitrogen) Triton X100 (97,063–864, VWR) 2-Mercaptoethanol (M3148, Sigma) 15 ml and 50 ml falcon tubes 1.5 ml eppendorf tubes Surgical fine forceps (RWD Life science) Surgical scissors (RWD Life science) ..
Microscopy:Article Title: Stable, neuron-specific gene expression in the mouse brain
Article Snippet: .. 10X PBS (1610780EDU, BioRad) 10X TBS (1706435EDU, BioRad) Tween-20 10% Solution (1610781EDU, BioRad) Ketamine (VINB-KETO-70121, Covetrus) Xylazine (AH0006P, Rampun) Heparin (H3393-100KU, Sigma) Syringe (701RN, Hamilton) Needles (Customized part #7803–07, Hamilton, 30G/15 mm/point 4/20° angle) VANNAS spring scissors (S11002-08, RWD) Small spatula 4% paraformaldehyde (J19943.K2, Life Technology Corporation) Trizol reagent (15,596,018, Life Technology Corporation) Ethanol 200 Proof (C933N40, Thomas Scientific) Isopropanol (BP26181, Thermofisher Scientific) First-strand cDNA synthesis master mix 4X (MB6008, ScienCell) Primers (IDT, see Table ) Powerup SYBR Green Master Mix (A25778, Applied Biosystems) PCR plates reader (Thermofisher Scientific) Pipettes and tips Guanidine-HCL (G4505, Sigma) Urea (U0631, Sigma) SDS (151–21-3, Bioworld) PBS-T (0.1%Tween-20 in PBS) 1% Casein in PBS or 5% non-fat milk in PBS (M-0842, Labscientific) O.C.T. compound (4583, Sakura) DAPI-prolonged diamond in mounting medium (P36962, Invitrogen) 4X Laemmli sample buffer (1,610,747, BioRad) Halt protease and phosphatase inhibitor cocktails (PI78446, Thermofisher scientific) Primary and secondary antibodies (see Table ) PM2500 protein marker (PM25002112201-1, ExcelBand) MES-SDS running buffer (NP0002, Invitrogen) HyPure Molecular Biology Grade Water (SH30538.03, VWR) Chloroform (LC130402, LabChem) MicroAmp Optical adhesive film (4,311,971, Applied Biosystems) Superfrost Plus Microscope Slides (22–037-246, Fisherbrand) NuPAGE 4–12% Bis–Tris Gel (NP0335B0X, Invitrogen) iBlot 2 NC Mini Stacks (IB23002, Invitrogen) Triton X100 (97,063–864, VWR) 2-Mercaptoethanol (M3148, Sigma) 15 ml and 50 ml falcon tubes 1.5 ml eppendorf tubes Surgical fine forceps (RWD Life science) Surgical scissors (RWD Life science) ..
other:
Article Title: Deconvolving organogenesis in space and time via spatial transcriptomics in thick tissues
Article Snippet: DMSO, anhydrous (Biosciences, #786-1666), 10x PBS, pH 7.8 (Bio-Rad, #1610780), 10x PBS, pH 7.4 (Quality Biological, #119-069-131), 1M Tris-HCl, pH 8.0 (Quality Biological, #351-007-101), 20% SDS (Research Products Internatioal, #151-21-3), 1M Tris, pH 7.0 (Invitrogen, #AM9850G), 0.5M EDTA, pH 8.0 (Invitrogen, #AM9260G), Triton X-100 (Sigma-Aldrich, #T8787-100ML), Glycerol (Fisher BioReagents, #BP229-1), Ethanol (Fisher BioReagents, #BP2818-500), Acetic Acid (Sigma-Aldrich, #A6283-500ML), Formamide (Sigma-Aldrich, #47671-250ML-F), Sucrose (Sigma-Aldrich, #S7903-1KG), O.C.T. compound (Tissue-Tek, #4583), Methanol (Sigma-Aldrich, #179337-1L), 20x SSC (Promega, #V4261), VWR Micro Slides (VWR North American, #48300-026), Cover Glass (Corning, #2850-18), Microseal B seal (Bio-Rad, MSB1001), GlassBottom Dish (Cellvis, D60-30-1.5-N), 16% Formaldehyde(Thermo Scientific, #28908), 1⁄2 ml Syringe (BD, #305620), PMSF (Cell Signaling Technology, #8553S), DNase I and DNA Digestion Buffer (New England Biolabs, #M0303L), Salmon Sperm DNA (Invitrogen, AM9680), Proteinase K (New England Biolabs, #P8107S), SplintR and SplintR ligase Reaction Buffer (New England Biolabs, #M0375L), Phi29 DNA Polymerase and Phi29 DNA Polymerase Reaction Buffer (New England Biolabs, #M0269L), Thermostable Inorganic Pyrophosphatase (New England Biolabs,, #M0296L), RNase Inhibitor Murine (New England Biolabs, #RNase Inhibitor Murine), Acryloyl-X (Invitrogen, #A20770), Bind Silane (Millipore Sigma, #M6514-25ML), Acrylamide (Bio-Rad, #1610140), Bis-Acrylamide (Bio-Rad, #1610142), N,N,N’,N’-Tetramethylethylenediamine (TEMED, Sigma-Aldrich, #T7024-50ML), Ammonium Persulfate (APS, Sigma-Aldrich, #A3678-100G), Deoxynucleotide (dNTP, New England Biolabs, #N0447S), N,N’-(1,2-Dihydroxyethylene)bisacrylamide (Trolox, Sigma-Aldrich, #294381-5G), Beta-mercaptoethanol (Sigma-Aldrich, #M6250), 5-AA-2’-dUTP (TriLink, N-2049), DAPI (Invitrogen, #D1306).
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